Purification and characterization of a tRNA nucleotidyltransferase from Lupinus albus and functional complementation of a yeast mutation by the corresponding cDNA

@article{Shanmugam2004PurificationAC,
  title={Purification and characterization of a tRNA nucleotidyltransferase from Lupinus albus and functional complementation of a yeast mutation by the corresponding cDNA},
  author={K. Shanmugam and P. J. Hanic-Joyce and P. Joyce},
  journal={Plant Molecular Biology},
  year={2004},
  volume={30},
  pages={281-295}
}
ATP (CTP):tRNA nucleotidyltransferase (EC 2.7.7.25) was purified to apparent homogeneity from a crude extract of Lupinus albus seeds. Purification was accomplised using a multistep protocol including ammonium sulfate fractionation and chromatography on anion-exchange, hydroxylapatite and affinity columns. The lupin enzyme exhibited a pH optimum and salt and ion requirements that were similar to those of tRNA nucleotidyltransferases from other sources. Oligonucleotides, based on partial amino… Expand
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