Purification and characterization of a pectin lyase produced by Curvularia inaequalis NRRL 13884 on orange peels waste , solid state culture

@inproceedings{Afifi2002PurificationAC,
  title={Purification and characterization of a pectin lyase produced by Curvularia inaequalis NRRL 13884 on orange peels waste , solid state culture},
  author={Alaa Afifi and Eman Mohammed Fawzi and M A Foaad},
  year={2002}
}
Utilization of orange peels as an agroindustrial waste for production of pectin lyase (PL) [E.C.4.2.2.10] by Curvularia inaequalis (Shear) Boedijn NRRL 13884 was investigated using solid state culture (SSC). The highest level of extracellular pectin lyase was detected with this waste as an inducing substrate. The enzyme was purified using Sephadex G-100 and DEAE-Cellulose column chromatography. It was purified about 50 fold. The enzyme optimum activity was at pH 5.0 and 45 °C. The enzyme was… CONTINUE READING

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