Purification and characterization of a novel β-agarase of Paenibacillus sp. SSG-1 isolated from soil.

@article{Song2014PurificationAC,
  title={Purification and characterization of a novel β-agarase of Paenibacillus sp. SSG-1 isolated from soil.},
  author={Tao Song and Yu Cao and Hui Xu and Weijia Zhang and Baojin Fei and Dairong Qiao and Y. W. Cao},
  journal={Journal of bioscience and bioengineering},
  year={2014},
  volume={118 2},
  pages={125-9}
}
Agar is a polysaccharide polymer material, generally extracted from seaweed. Most agar degradation strains were isolated from seawater. In order to find new species resources and novel agarase from soil, an agar-degrading bacterium Paenibacillus sp. SSG-1 was isolated from soil. Agarase SSG-1a was purified to homogeneity by 30.2 fold with a yield of 4.8% through ammonium sulfate precipitation, DEAE FF chromatography and native-PAGE separation. The tandem mass spectrometry (MS/MS) results… CONTINUE READING

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