Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella.

@article{Zhao2009PurificationAC,
  title={Purification and characterization of a novel lectin from the toxic wild mushroom Inocybe umbrinella.},
  author={Jie Zhao and Hexiang Wang and T. B. Ng},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2009},
  volume={53 3},
  pages={
          360-6
        }
}
From the dried fruiting bodies of the toxic mushroom Inocybe umbrinella, a novel lectin with a molecular mass of 17 kDa has been isolated with about 160-fold purification. The purification protocol comprised ion exchange chromatography on DEAE-cellulose, and CM-cellulose, and gel filtration on Superdex 75. Among the carbohydrates tested, raffinose, d-melibiose, alpha-lactose and d(+)-galactose could inhibit the hemagglutinating activity of the lectin. The hemagglutinating activity was stable… CONTINUE READING
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