Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp. nov. KRF1T

@article{Yuan2012PurificationAC,
  title={Purification and characterization of a novel extracellular inulinase from a new yeast species Candida kutaonensis sp. nov. KRF1T},
  author={Bo Yuan and Nan Hu and Juan Sun and Shi-an Wang and Fuli Li},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={96},
  pages={1517-1526}
}
A novel extracellular exoinulinase was purified and characterized from a new yeast strain KRF1T, and the gene encoding the enzyme was successfully cloned. The enzyme was stable at low pH between 3.0 and 6.5. The K m and V max values of the purified enzyme for inulin were 2.3 mg/mL and 4.8 mg/min, respectively. The optimum temperature of the inulinase was 50 °C, and the enzyme remained 78 % of activity at 60 °C for 2 h. The inulinase showed an amino acid sequence identity of 58 % to its closest… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-7 of 7 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 52 references

Purification and characterisation of exo- and endo-inulinase from Aspergillus ficuum JNSP5-06

HQ Chen, XM Chen, +6 authors ZJ Xie
Food Chem • 2009
View 5 Excerpts
Highly Influenced

Purification and characterization of extracellular inulinase from a marine yeast Pichia guilliermondii and inulin hydrolysis by the purified inulinase

F Gong, T Zhang, +3 authors XH Wang
Biotechnol Bioproc Eng • 2008
View 7 Excerpts
Highly Influenced

Optimization of fermentation conditions for the biosynthesis of inulinase by the new source; Aspergillus tamarii and hydrolysis of some inulin containing agrowastes

WIA Saber, NE El-Naggar
Biotechnology • 2009
View 2 Excerpts
Highly Influenced

Crystallization and general properties of an extracellular inulinase from Aspergillus sp

T Nakamura, T Kurokawa, S Nakatsu, S Ueda
Nippon Nogeikagaku Kaishi • 1978
View 2 Excerpts
Highly Influenced

Algur OF (2011) Inulinase production by Geotrichum candidum using Jerusalem artichoke as sole carbon source

S Erdal, O Canli
2011

Similar Papers

Loading similar papers…