Purification and characterization of a novel cysteine synthase isozyme from spinach hydrated seeds.


A novel type of cysteine synthase (CSase, EC isozyme, designated as CSase 1', was purified to homogeneity from hydrated spinach seeds. The enzyme had a molecular weight of 68,000 and consisted of two identical subunits of M(r), 34,000. The apparent K(m) for O-acetyl-L-serine was 8.33 mM and that for sulfide was 0.66 mM. The activity of CSase 1… (More)


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