Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25.


A novel exo-beta-mannanase (1,4-beta-D-mannan mannobiohydrolase) was isolated from the culture fluid of strain No. F-25 of Aeromonas hydrophila subspecies anaerogenes, and purified about 4,000-fold by ammonium sulfate precipitation and successive co.umn chromatographies. The final enzyme preparation appeared to be homogeneous on polyacrylamide gel… (More)