Purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium Paenibacillus polymyxa EJS-3.

@article{Lu2010PurificationAC,
  title={Purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium Paenibacillus polymyxa EJS-3.},
  author={Fengxia Lu and Zhaoxin Lu and Xiaomei Bie and Zhengying Yao and Yufeng Wang and Yaping Lu and Yao Guo},
  journal={Thrombosis research},
  year={2010},
  volume={126 5},
  pages={e349-55}
}
INTRODUCTION Endophytes may become a new source of thrombolytic agents for thrombosis treatment. MATERIALS AND METHODS A novel fibrinolytic enzyme from Paenibacillus polymyxa EJS-3 (PPFE-I) was purified with ammonium sulfate precipitation, hydrophobic chromatography, ion exchange and gel filtration chromatography. The characterization of the enzyme was… CONTINUE READING