Purification and characterization of a novel histone H2A specific protease (H2Asp) from chicken liver nuclear extract.

@article{Panda2013PurificationAC,
  title={Purification and characterization of a novel histone H2A specific protease (H2Asp) from chicken liver nuclear extract.},
  author={Pragnya Panda and Madan Mohan Chaturvedi and Amulya Kumar Panda and Mrutyunjay Suar and Jogeswar Satchidananda Purohit},
  journal={Gene},
  year={2013},
  volume={512 1},
  pages={47-54}
}
The proteolysis of the N- or the C-terminal tails of histones have recently emerged as a novel form of irreversible posttranslational modifications of histones. However, there are very few reports describing purification of a histone specific protease. Here, we report a histone H2A specific protease (H2Asp) activity in the chicken liver nuclear extract. The H2Asp was purified to homogeneity and was found to be a ~10.5kDa protein. It demonstrated high specificity to histone H2A and was an… CONTINUE READING