Purification and characterization of a nitrilase from Aspergillus niger K10

  title={Purification and characterization of a nitrilase from Aspergillus niger K10},
  author={Ondřej Kaplan and Vojtěch Vejvoda and Ondřej Pl{\'i}hal and Petr Pompach and Daniel Kavan and Pavla Bojarov{\'a} and Karel Bezou{\vs}ka and Martina Mackov{\'a} and Maria Cantarella and Vladim{\'i}r Jirků and Vladim{\'i}r Křen and Ludmila Mart{\'i}nkov{\'a}},
  journal={Applied Microbiology and Biotechnology},
Aspergillus niger K10 cultivated on 2-cyanopyridine produced high levels of an intracellular nitrilase, which was partially purified (18.6-fold) with a 24% yield. The N-terminal amino acid sequence of the enzyme was highly homologous with that of a putative nitrilase from Aspergillus fumigatus Af293. The enzyme was copurified with two proteins, the N-terminal amino acid sequences of which revealed high homology with those of hsp60 and an ubiquitin-conjugating enzyme. The nitrilase exhibited… CONTINUE READING
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