Purification and characterization of a new type of glutamine synthetase from cyanobacteria.

@article{GarcaDomnguez1997PurificationAC,
  title={Purification and characterization of a new type of glutamine synthetase from cyanobacteria.},
  author={Mario Garc{\'i}a-Dom{\'i}nguez and Jos{\'e} C. Reyes and Francisco J. Florencio},
  journal={European journal of biochemistry},
  year={1997},
  volume={244 1},
  pages={
          258-64
        }
}
The cyanobacterium Synechocystis sp. PCC 6803 contains two genes encoding two different types of glutamine synthetases (GS), glnA and glnN. The first codes for a typical prokaryotic GS type I and the second one codes for a GS type III, different in amino acid sequence to the prokaryotic GSI and the eukaryotic GSII. The glnN gene has been expressed in Escherichia coli and the corresponding protein purified almost to homogeneity (92%). The native enzyme (500 kDa) was composed of six identical… 
Purification and characterization of glutamine synthetase from the unicellular cyanobacterium Synechococcus RF-1
TLDR
Glutamine synthetase (GS; EC 6.3.2) from the Synechococcus RF-1 was purified to homogeneity by ion exchange, molecular sieving, and hydroxyapatite chromatographies, and Electron micrographs of the enzyme revealed two parallel protein layers in cubic symmetry with quaternary structure.
Biochemical and Mutational Analysis of Glutamine Synthetase Type III from the Rumen Anaerobe Ruminococcus albus 8
ABSTRACT Two different genes encoding glutamine synthetase type I (GSI) and GSIII were identified in the genome sequence of R. albus 8. The identity of the GSIII protein was confirmed by the presence
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TLDR
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Function and Regulation of glnA in the Methanogenic Archaeon Methanococcus maripaludis
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The Presence of GSI-Like Genes in Higher Plants: Support for the Paralogous Evolution of GSI and GSII Genes
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Ammonium assimilation in cyanobacteria
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Cyanobacteria perceive nitrogen status by sensing the intracellular concentration of 2-oxoglutarate, a signaling metabolite that is able to modulate allosterically the function of NtcA, in vitro and in vivo.
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Recombinant GMAS had the same properties as intrinsic GMAS, and it formed theanine by coupling the reaction with the ATP-regeneration system of yeast sugar fermentation.
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TLDR
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TLDR
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TLDR
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