Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis.

@article{Shrimankar1992PurificationAC,
  title={Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis.},
  author={Paresh Shrimankar and L Stordal and Russell Maurer},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 23},
  pages={7689-96}
}
The dnaB gene of Escherichia coli encodes an essential DNA replication enzyme. Fueled by the energy derived from the hydrolysis of ATP to ADP+P(i), this enzyme unwinds double-stranded DNA in advance of the DNA polymerase. While doing so, it intermittently stimulates primase to synthesize an RNA primer for an Okazaki fragment. To better understand the structural basis of these and other aspects of DnaB function, we have initiated a study of mutant DnaB proteins. Here, we report the purification… CONTINUE READING