Purification and characterization of a milk clotting protease from Rhizomucor miehei

@article{Preetha1997PurificationAC,
  title={Purification and characterization of a milk clotting protease from Rhizomucor miehei},
  author={S. Preetha and Rathanam Boopathy},
  journal={World Journal of Microbiology and Biotechnology},
  year={1997},
  volume={13},
  pages={573-578}
}
  • S. Preetha, R. Boopathy
  • Published 1 September 1997
  • Chemistry
  • World Journal of Microbiology and Biotechnology
Benzamidine, an inhibitor of serine proteases, was used as an affinity ligand for the purification of aspartyl protease from culture filtrate of Rhizomucor miehei. The two step purification protocol (ion-exchange and affinity chromatography) resulted in a homogenous enzyme preparation with seven-fold purification and a final recovery of 22%. The purified enzyme was free of brown pigmentation, a factor inherently associated with the enzyme; it was stable and active at acidic pH (optimum pH 4.1… 

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