Purification and characterization of a milk clotting protease from Rhizomucor miehei

@article{Preetha1997PurificationAC,
  title={Purification and characterization of a milk clotting protease from Rhizomucor miehei},
  author={S. Preetha and Rathanam Boopathy},
  journal={World Journal of Microbiology and Biotechnology},
  year={1997},
  volume={13},
  pages={573-578}
}
Benzamidine, an inhibitor of serine proteases, was used as an affinity ligand for the purification of aspartyl protease from culture filtrate of Rhizomucor miehei. The two step purification protocol (ion-exchange and affinity chromatography) resulted in a homogenous enzyme preparation with seven-fold purification and a final recovery of 22%. The purified enzyme was free of brown pigmentation, a factor inherently associated with the enzyme; it was stable and active at acidic pH (optimum pH 4.1… Expand
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