Purification and characterization of a major zinc binding protein from renal brush border membrane of rat.

@article{Kumar1999PurificationAC,
  title={Purification and characterization of a major zinc binding protein from renal brush border membrane of rat.},
  author={Rajiv Ranjan Kumar and Rajendra V V S Prasad},
  journal={Biochimica et biophysica acta},
  year={1999},
  volume={1419 1},
  pages={23-32}
}
In spite of the fact that zinc is an essential trace element, mechanisms that contribute to zinc homeostasis in mammals are poorly understood. An attempt has been made to identify and purify zinc binding components from renal brush border membrane (BBM), which could be involved in the binding of zinc and the subsequent translocation across the BBM. A 40 kDa major zinc binding protein has been identified and purified from renal BBM, which showed a dissociation constant (Kd) of 211 microM and… CONTINUE READING