Purification and characterization of a major allergen from the house dust mite Dermatophagoides farinae.

@article{Holck1986PurificationAC,
  title={Purification and characterization of a major allergen from the house dust mite Dermatophagoides farinae.},
  author={Askild Holck and Susan Dale and Knut Sletten},
  journal={Allergy},
  year={1986},
  volume={41 6},
  pages={408-17}
}
A major allergen from an extract of the house dust mite, Dermatophagoides farinae, was shown to be extremely heterogenic with respect to charge. A slightly basic component of this allergen with a pI of 8, was purified by isoelectric focusing in two steps. The purified component, denoted antigen 19/20 IIa, seemed to be representative for the allergenic activity of the major allergen. The amino acid analysis suggested that antigen 19/20 IIa had a molecular weight of 9400 and contained one residue… CONTINUE READING

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