• Chemistry, Medicine
  • Published in
    Journal of agricultural and…
    1999
  • DOI:10.1021/jf980853z

Purification and characterization of a lipoxygenase enzyme from durum wheat semolina.

@article{Barone1999PurificationAC,
  title={Purification and characterization of a lipoxygenase enzyme from durum wheat semolina.},
  author={Ren{\'e} Barone and Raffaella Briante and Sabato d'Auria and Ferdinando Febbraio and Carlo Vaccaro and Luigi Del Giudice and Grazia M Borrelli and Natale Di Fonzo and Roberto Nucci},
  journal={Journal of agricultural and food chemistry},
  year={1999},
  volume={47 5},
  pages={
          1924-31
        }
}
Purification of a lipoxygenase enzyme from the cultivar Tresor of durum wheat semolina (Triticum turgidum var. durum Desf) was reinvestigated furnishing a new procedure. The 895-fold purified homogeneous enzyme showed a monomeric structure with a molecular mass of 95 +/- 5 kDa. Among the substrates tested, linoleic acid showed the highest k(cat)/K(m) value; a beta-carotene bleaching activity was also detected. The enzyme optimal activity was at pH 6. 8 on linoleic acid as substrate and at pH 5… CONTINUE READING

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