Purification and characterization of a human factor that assembles and remodels chromatin.

Abstract

We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is DNA-dependent and nucleosome-stimulated. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates. In addition to hSNF2h, this complex also contains a 190-kDa protein encoded by the BAZ1A gene. Since both subunits are homologues of the Drosophila ACF complex (ATP-utilizing chromatin assembly and remodeling factor), we have named this factor human ACF or hACF.

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@article{Leroy2000PurificationAC, title={Purification and characterization of a human factor that assembles and remodels chromatin.}, author={Gary Leroy and Alejandra Loyola and William S. Lane and Danny F Reinberg}, journal={The Journal of biological chemistry}, year={2000}, volume={275 20}, pages={14787-90} }