Purification and characterization of a cadmium-induced metallothionein from the shore crab Carcinus maenas (L.).

@article{Pedersen1994PurificationAC,
  title={Purification and characterization of a cadmium-induced metallothionein from the shore crab Carcinus maenas (L.).},
  author={Kim Pedersen and S\oren N Pedersen and Peter H\ojrup and Jens Skorstengaard Andersen and Peter Roepstorff and Jens Knudsen and Michael Depledge},
  journal={The Biochemical journal},
  year={1994},
  volume={297 ( Pt 3)},
  pages={609-14}
}
Two metallothionein variants were purified from the midgut gland of crabs (Carcinus maenas) exposed to a high cadmium concentration (2 p.p.m.). One of the variants was purified from crabs exposed to a low cadmium concentration (0.5 p.p.m.). The purification method involved acetone precipitation, gel filtration and reversed-phase h.p.l.c. The complete amino acid sequences of both variants have been elucidated by m.s. and automated sequence analysis on S-methylated proteins or fragments produced… CONTINUE READING