Purification and characterization of a nylon-degrading enzyme.

@article{Deguchi1998PurificationAC,
  title={Purification and characterization of a nylon-degrading enzyme.},
  author={Tetsuya Deguchi and Yoshihisa Kitaoka and M Kakezawa and Tomoaki Nishida},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 4},
  pages={1366-71}
}
A nylon-degrading enzyme found in the extracellular medium of a ligninolytic culture of the white rot fungus strain IZU-154 was purified by ion-exchange chromatography, gel filtration chromatography, and hydrophobic chromatography. The characteristics of the purified protein (i.e., molecular weight, absorption spectrum, and requirements for 2,6-dimethoxyphenol oxidation) were identical to those of manganese peroxidase, which was previously characterized as a key enzyme in the ligninolytic… CONTINUE READING

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