Purification and characterization of a puromycin-hydrolyzing enzyme from blasticidin S-producing Streptomyces morookaensis.

@article{Nishimura1998PurificationAC,
  title={Purification and characterization of a puromycin-hydrolyzing enzyme from blasticidin S-producing Streptomyces morookaensis.},
  author={Motohiro Nishimura and Hisayuki Matsuo and Ayako Nakamura and Masanori Sugiyama},
  journal={Journal of biochemistry},
  year={1998},
  volume={123 2},
  pages={247-52}
}
Blasticidin S-producing Streptomyces morookaensis JCM4673 produces an enzyme which inactivates puromycin (PM) by hydrolyzing an amide linkage between its aminonucleoside and O-methyl-L-tyrosine moieties [Nishimura et al. (1995) FEMS Microbiol. Lett. 132, 95-100]. In this study, we purified to homogeneity the enzyme from the cell-free extracts of S… CONTINUE READING