Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua).

@article{Lanes2000PurificationAC,
  title={Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua).},
  author={Olav Lanes and P H Guddal and Dag Rune Gjellesvik and Nils Peder Willassen},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2000},
  volume={127 3},
  pages={399-410}
}
Uracil-DNA glycosylase (UDG; UNG) has been purified 17000-fold from Atlantic cod liver (Gadus morhua). The enzyme has an apparent molecular mass of 25 kDa, as determined by gel filtration, and an isoelectric point above 9.0. Atlantic cUNG is inhibited by the specific UNG inhibitor (Ugi) from the Bacillus subtilis bacteriophage (PBS2), and has a 2-fold higher activity for single-stranded DNA than for double-stranded DNA. cUNG has an optimum activity between pH 7.0-9.0 and 25-50 mM NaCl, and a… CONTINUE READING