Purification and characterization of a DNase gamma-like endonuclease from Xenopus laevis liver.

@article{Umemori1998PurificationAC,
  title={Purification and characterization of a DNase gamma-like endonuclease from Xenopus laevis liver.},
  author={Kiyoko Umemori and Atsushi Nishikawa and Sei-ichi Tanuma},
  journal={Apoptosis : an international journal on programmed cell death},
  year={1998},
  volume={3 3},
  pages={145-53}
}
We recently found that two apoptotic DNase gamma-like endonucleases (36 and 38 kDa DNases) were present in Xenopus laevis larval and adult liver cell nuclei and that their activities increased in metamorphic climax. Here, we purified the main DNase gamma-like endonuclease from Xenopus laevis liver cell nuclei and characterized its physical and enzymatic properties in detail. The molecular mass of Xenopus liver nuclear endonuclease was 38,000 daltons as determined by SDS-polyacrylamide gel… CONTINUE READING