Purification and characterization of a DNA-dependent RNA polymerase from vaccinia virions.

Abstract

A DNA-dependent RNA polymerase has been extracted from vaccinia virions and purified to near homogeneity as judged by glycerol gradient sedimentation and polyacrylamide gel electrophoresis. The native enzyme has a molecular weight of approximately 500,000 and can be dissociated into putative subunits of 140,000, 137,000, 37,000, 35,000, 31,000, 22,000, and… (More)

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@article{Baroudy1980PurificationAC, title={Purification and characterization of a DNA-dependent RNA polymerase from vaccinia virions.}, author={B. M. Baroudy and Bernard Moss}, journal={The Journal of biological chemistry}, year={1980}, volume={255 9}, pages={4372-80} }