Purification and characterization of a phenoloxidase (laccase) from the lignin-degrading basidiomycete PM1 (CECT 2971).

@article{Coll1993PurificationAC,
  title={Purification and characterization of a phenoloxidase (laccase) from the lignin-degrading basidiomycete PM1 (CECT 2971).},
  author={Pedro M. Coll and Jos{\'e} Manuel Fern{\'a}ndez-Abalos and Julio R. Villanueva and Ram{\'o}n I Santamar{\'i}a and Patricia P{\'e}rez},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 8},
  pages={2607-13}
}
A new lignin-degrading basidiomycete, strain PM1 (= CECT 2971), was isolated from the wastewater of a paper factory. The major ligninolytic activity detected in the basidiomycete PM1 culture supernatant was a phenoloxidase (laccase). This activity was produced constitutively in defined or complex media and appeared as two protein bands in native gel electrophoresis preparations. No enzyme induction was found after treatment with certain potential laccase inducers. Laccase I was purified to… CONTINUE READING

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