Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori).

Abstract

NADH-dependent glutamate synthase (Nadh-Gogat; EC 1.41.14) was purified 766-fold from the fat body of 5th instar larvae of the silkworm with a final specific activity of 13.8 units/mg protein by a produce including ammonium sulfate fraction, Q-Sepharose HP ion exchange column chromatography, Blue Sepharose FF affinity column chromatography and Superdex 200… (More)

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@article{Hirayama1998PurificationAC, title={Purification and characterization of NADH-dependent glutamate synthase from the silkworm fat body (Bombyx mori).}, author={Chuichi Hirayama and H. Saito and Kuwayama T Konno and Hiromi Shinbo}, journal={Insect biochemistry and molecular biology}, year={1998}, volume={28 7}, pages={473-82} }