Purification and characterization of HIV-human protein complexes.

@article{Jger2011PurificationAC,
  title={Purification and characterization of HIV-human protein complexes.},
  author={Stefanie J{\"a}ger and Natali Gulbahce and Peter Cimermancic and Joshua Kane and Nanhai He and Seemay Chou and Iv{\'a}n D'Orso and Jason Fernandes and Gwendolyn M. Jang and Alan D. Frankel and Tom Alber and Qiang Zhou and Nevan J. Krogan},
  journal={Methods},
  year={2011},
  volume={53 1},
  pages={13-9}
}
To fully understand how pathogens infect their host and hijack key biological processes, systematic mapping of intra-pathogenic and pathogen-host protein-protein interactions (PPIs) is crucial. Due to the relatively small size of viral genomes (usually around 10-100 proteins), generation of comprehensive host-virus PPI maps using different experimental platforms, including affinity tag purification-mass spectrometry (AP-MS) and yeast two-hybrid (Y2H) approaches, can be achieved. Global maps… CONTINUE READING