Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli.

@article{Voordouw1987PurificationAC,
  title={Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli.},
  author={Gerrit Voordouw and Wilfred R. Hagen and K M Kr{\"u}se-Wolters and A van Berkel-Arts and Cees Veeger},
  journal={European journal of biochemistry},
  year={1987},
  volume={162 1},
  pages={31-6}
}
Hydrogenase from Desulfovibrio vulgaris (Hildenborough) is a heterologous dimer of molecular mass 46 + 13.5 kDa. Its two structural genes have been cloned on a 4664-base-pair fragment of known sequence in the vector pUC9. Expression of hydrogenase polypeptides in Escherichia coli transformed with this plasmid is poor (approximately 0.1% w/w of total protein). Deletion of up to 1.9 kb of insert DNA brings the gene encoding for the large subunit in close proximity to the lac promotor of pUC9 and… CONTINUE READING

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