Purification and characterization of ATP:citrate lyase from Hydrogenobacter thermophilus TK-6.


ATP:citrate lyase [ATP citrate (pro-3S)-lyase; EC] was purified and characterized from the cells of Hydrogenobacter thermophilus, an aerobic, thermophilic, hydrogen-oxidizing bacterium which fixes carbon dioxide by a reductive carboxylic acid cycle. The enzyme was quite stable, even in the absence of sulfhydryl reagents. Optimum pH for reaction was… (More)


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