Purification and characterization of β-glucosidase fromMelanocarpus sp. MTCC 3922

@inproceedings{Kaur2007PurificationAC,
  title={Purification and characterization of β-glucosidase fromMelanocarpus sp. MTCC 3922},
  author={Jatinder Kaur and Bhupinder Singh Chadha and K. B. Aneesh Kumar and Sonia Ghatora and Harvinder S. Saini},
  year={2007}
}
This study reports the purification and characterization of β-glucosidase from a newly isolated thermophilic fungus, Melanocarpus sp. Microbial Type Culture Collection (MTCC) 3922. The molecular weight of β-glucosidase was determined to be ~ 92 and 102 kDa with SDS PAGE and gel filtration, respectively, and pI of ~ 4.1. It was optimally active at 60°C and pH 6.0, though was stable at 50°C and pH 5.0 - 6.0. The presence of DTT, mercaptoethanol and metal ions such as Na+, K+, Ca2+, Mg2+ and Zn2… CONTINUE READING

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