• Chemistry
  • Published 2004

Purification and characterization fo bovine liver #beta#-D-Glucuronidase

@inproceedings{Feng2004PurificationAC,
  title={Purification and characterization fo bovine liver #beta#-D-Glucuronidase},
  author={Bo Feng and Min Wang and Min Fang},
  year={2004}
}
Using means of autolysis,35% saturated ammonium sulfate fractionation,CM-cellulose column chromatography,and Hydroxyapatite chromatography to purify #beta#-D-GLucuronidase from bovine liver,an 42% yield of enzyme with a specific activity of approximately 26700U/mg was obtained.The purified enzyme shows signle band on SDS-PAGE.The optimum pH is 5.5,and the enzyme was most stable from pH5.0 to pH6.0.THe K_(m) with phenolphthalein glucuronide and nitrophenyl grlucuronide as substrates were 0.2… CONTINUE READING

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