Purification and cDNA cloning of an insect defensin from larvae of the longicorn beetle, Acalolepta luxuriosa

  title={Purification and cDNA cloning of an insect defensin from larvae of the longicorn beetle, Acalolepta luxuriosa},
  author={Kenjiro Ueda and Morikazu Imamura and Ayaka Saito and Ryoichi Sato},
  journal={Applied Entomology and Zoology},
We purified an antibacterial peptide from the larval hemolymph of a coleopteran beetle, Acalolepta luxuriosa. Using structure analysis and cDNA cloning, this peptide was identified as a novel member of the insect defensin family and named A. luxuriosa defensin 1. A. luxuriosa defensin 1 shares high sequence similarity with other defensins, especially coleopteran defensins. A dendrogram of coleopteran insect defensins based on sequence homology revealed that A. luxuriosa defensin is closer to… 

Figures and Tables from this paper

Identification, phylogenetic analysis and expression profile of an anionic insect defensin gene, with antibacterial activity, from bacterial-challenged cotton leafworm, Spodoptera littoralis

An important immune role of the defensin gene in Spodoptera littoralis is highlighted by cooperating with other AMPs to control bacterial infection by integrating sequence analyses of the data.

Bactrocerin-1: a novel inducible antimicrobial peptide from pupae of oriental fruit fly Bactrocera dorsalis Hendel.

Minimal growth inhibition concentration measurements for synthesized Bactrocerin-1 showed a very broad spectrum of anti-microbial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi.

Isolation, Identification, and Bioinformatic Analysis of Antibacterial Proteins and Peptides from Immunized Hemolymph of Red Palm Weevil Rhynchophorus ferrugineus

Red palm weevil (Rhynchophorus ferrugineus Olivier, 1791, Coleoptera: Curculionidae) is a destructive pest of palms, rapidly extending its native geographical range and causing large economic losses

A bioinformatic study of antimicrobial peptides identified in the Black Soldier Fly (BSF) Hermetia illucens (Diptera: Stratiomyidae)

The larvae and the combined adult male and female H. illucens transcriptomes were examined, and all the sequences, putatively encoding AMPs, were analysed with different machine learning-algorithms to identify 57 putatively active peptides suitable for subsequent experimental validation studies.

Insect antimicrobial peptides and their applications

Current knowledge and recent progress in several classes of insect AMPs, including insect defensins, cecropins, attacins, lebocins and other proline-rich peptides, gloverins, and moricins are discussed, with a focus on structural-functional relationships and their potential applications.

Pharmaco-medical Application of Antimicrobial Peptides Derived from Insect

This review focuses on AMPs from insects and discusses current knowledge and recent progress with potential applications of insect AMPs.

Structural and Functional Characterization of a Novel Recombinant Antimicrobial Peptide from Hermetia illucens

This work investigates a defensin-like antimicrobial peptide identified in the Hermetia illucens insect through a combination of transcriptomics and bioinformatics approaches, and suggests disruption of the cell membrane architecture and pore formation with leaking of cytosolic material.

Convergent evolution in the antennae of a cerambycid beetle, Onychocerus albitarsis, and the sting of a scorpion

The first known case of a cerambycid beetle using its antennae to inject a secretion that causes cutaneous and subcutaneous inflammation in humans is reported, which is almost identical to that found in the stinger of a deadly buthid scorpion.



Purification, cDNA cloning and modification of a defensin from the coconut rhinoceros beetle, Oryctes rhinoceros.

A novel member of the insect defensins, a family of antibacterial peptides, was purified from larvae of the coconut rhinoceros beetle, Oryctes rh inoceros, immunized with Escherichia coli and showed strong antibacterial activity against Staphylococcus aureus.

Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvae of the coleopteran, Tenebrio molitor.

Antibacterial activity was induced in the hemolymph of larvae of the coleopteran Tenebrio molitor by injection of Escherichia coli using an antibacterial protein, named tenecin 1, which was found to inhibit the growth of Gram-positive bacteria.

Isolation and characterization of a new member of the insect defensin family from a beetle, Allomyrina dichotoma.

Analysis of the antibacterial spectrum of A. dichotoma defensin indicated that this peptide showed antibacterial activity against Gram-positive bacteria like Staphylococcus aureus and Bacillus subtilis but not against gram-negative bacteria like Escherichia coli and Pseudomonas aeruginosa, indicating a typical spectrum of the insectdefensin family.

Novel inducible antibacterial peptides from a hemipteran insect, the sap-sucking bug Pyrrhocoris apterus.

The first report on inducible antibacterial molecules in the sap-sucking bug Pyrrhocoris apterus, a representative species of the Hemiptera, which predated the Endoptergotes by at least 50 million years in evolution is presented.

Purification and molecular cloning of cDNA for an inducible antibacterial protein of larvae of a coleopteran insect, Holotrichia diomphalia.

An antibacterial protein was found to inhibit the growth of Gram-negative bacteria and to consist of 72-amino acid residues with no cysteine residues.

A novel insect defensin mediates the inducible antibacterial activity in larvae of the dragonfly Aeschna cyanea (Paleoptera, Odonata).

A 38-residue peptide is isolated from this hemolymph of dragonflies which is strongly active against Gram-positive bacteria and also shows activity against one of the Gram-negative bacteria which was tested.