Purification and cDNA cloning of a cecropin from the longicorn beetle, Acalolepta luxuriosa.

@article{Saito2005PurificationAC,
  title={Purification and cDNA cloning of a cecropin from the longicorn beetle, Acalolepta luxuriosa.},
  author={Ayaka Saito and Kenjiro Ueda and Morikazu Imamura and Shogo Atsumi and Hiroko Tabunoki and Nami Miura and Ayako Watanabe and Madoka Kitami and Ryoichi Sato},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2005},
  volume={142 3},
  pages={317-23}
}
We have cloned and characterized a novel antibacterial peptide from the hemolymph of the coleopteran insect Acalolepta luxuriosa, of the superfamily Cerambyocidea. This peptide is active against Micrococcus luteus and Escherichia coli, and the amino acid sequence deduced by cloning of the cDNA identifies it as a coleopteran cecropin. Sequence comparisons and phylogenetic analyses performed using Clustal X suggest that this cecropin is evolutionarily intermediate between dipteran and… CONTINUE READING

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