Purification and biosynthesis of cottonseed (Gossypium hirsutum L.) catalase.

  title={Purification and biosynthesis of cottonseed (Gossypium hirsutum L.) catalase.},
  author={Christine M. Kunce and Richard N. Trelease and Rickie B. Turley},
  journal={The Biochemical journal},
  volume={251 1},
As part of our research on peroxisome biogenesis, catalase was purified from cotyledons of dark-grown cotton (Gossypium hirsutum L.) seedlings and monospecific antibodies were raised in rabbits. Purified catalase appeared as three distinct electrophoretic forms in non-denaturing gels and as a single protein band (with a subunit Mr of 57,000) on silver-stained SDS/polyacrylamide gels. Western blots of crude extracts and isolated peroxisomes from cotton revealed one immunoreactive polypeptide… CONTINUE READING

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