Purification and biological characterization of shiga toxin from Shigella dysenteriae 1.

@article{Brown1982PurificationAB,
  title={Purification and biological characterization of shiga toxin from Shigella dysenteriae 1.},
  author={J. E. Brown and Diane E Griffin and Sara W. Rothman and Bhupendra P. Doctor},
  journal={Infection and immunity},
  year={1982},
  volume={36 3},
  pages={996-1005}
}
Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and preparative isoelectric focusing in sucrose gradients. The purified toxin was resolved by discontinuous polyacrylamide gel electrophoresis into a major cytotoxic protein band and a closely migrating… CONTINUE READING

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