Purification and biochemical properties of soluble recombinant human Bax.

  title={Purification and biochemical properties of soluble recombinant human Bax.},
  author={Sialyl Lewis and Susanne S. Bethell and Sheetal K Patel and J C Martinou and Bruno Antonsson},
  journal={Protein expression and purification},
  volume={13 1},
Bax is a member of the Bcl-2 protein family with proapoptotic properties. The proteins of this family contain three highly conserved regions termed BH1, BH2, and BH3 as well as a hydrophobic COOH-terminal domain, which is responsible for the membrane attachment of the proteins. We have expressed human Bax truncated of the 20 amino acid COOH-terminal hydrophobic domain to obtain large amounts of soluble protein suitable for biochemical and structural studies. The truncated protein was expressed… CONTINUE READING

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