Purification and biochemical properties of a galactooligosaccharide producing β-galactosidase from Bullera singularis

@article{Cho2003PurificationAB,
  title={Purification and biochemical properties of a galactooligosaccharide producing β-galactosidase from Bullera singularis},
  author={Youn-Jeung Cho and Hyun-Jae Shin and C. Bucke},
  journal={Biotechnology Letters},
  year={2003},
  volume={25},
  pages={2107-2111}
}
A β-galactosidase, catalyzing lactose hydrolysis and galactooligosaccharide (GalOS) synthesis from lactose, was extracted from the yeast, Bullera singularis KCTC 7534. The crude enzyme had a high transgalactosylation activity resulting in the oligosaccharide conversion of over 34% using pure lactose and cheese whey permeate as substrates. The enzyme was purified by two chromatographic steps giving 96-fold purification with a yield of 16%. The molecular weight of the purified enzyme (specific… CONTINUE READING

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