Purification and biochemical characterization of a fibroblast growth factor-binding protein (FGF-BP) from the lactoferrin fraction of bovine milk.

@article{Kawakami2006PurificationAB,
  title={Purification and biochemical characterization of a fibroblast growth factor-binding protein (FGF-BP) from the lactoferrin fraction of bovine milk.},
  author={Akio Kawakami and Kyoko Hirayama and Fumitaka Kawakami and Hiroshi Kawakami and Michio Fujihara and Kenzo Ohtsuki},
  journal={Biochimica et biophysica acta},
  year={2006},
  volume={1760 3},
  pages={421-31}
}
By means of gel filtration on a TSK-gel HPLC column in the presence of 8 M urea, a 37-kDa polypeptide (p37) was completely separated from lactoferrin (LF) in the heparin HII fraction of the partially purified LF fraction prepared from bovine milk. Purified p37 was identified as a fibroblast growth factor-binding protein (FGF-BP), since its N-terminal 14… CONTINUE READING