Purification and biochemical characterization of chicken anaemia agent.

@article{Todd1990PurificationAB,
  title={Purification and biochemical characterization of chicken anaemia agent.},
  author={Daniel Todd and Julie L Creelan and Dermot P Mackie and Frazer J. Rixon and M. S. Mcnulty},
  journal={The Journal of general virology},
  year={1990},
  volume={71 ( Pt 4)},
  pages={
          819-23
        }
}
Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA based on a CAA-specific monoclonal antibody. Virus particles banded at a density of 1.33 to 1.34 g/ml in CsCl and measured 23.5 +/- 0.8 nm in diameter. Purified preparations contained one major polypeptide (Mr 50,000) and a single-stranded, circular… Expand
Quantification of chicken anaemia virus by competitive polymerase chain reaction
TLDR
Quantification of CAV DNA molecules by the competitive PCR was rapid and highly reproducible when compared with conventional infectivity titration methods, suggesting the existence of different infection efficiencies to MDCC-MSB1 cells by isolates. Expand
Cloning and expression of chicken anemia virus VP3 protein in Escherichia coli.
TLDR
This finding indicates that recombinant VP3 expressed in the pTrcHis2 vector system can be used as antigen to detect anti-CAV antibodies. Expand
Chicken anemia agent: an electron microscopic study.
Particles of chicken anemia agent (CAA) negatively stained with uranyl acetate were found to be 26.5 nm in diameter. The surface detail evident on the particles indicated that the virus capsid wasExpand
Isolation and Identification of Chicken Infectious Anemia Virus in China
TLDR
Findings demonstrate that CIAV is present in China and could be demonstrated in SR43-infected MDCC-MSB-1 cells, a cell line derived from a Marek's disease lymphoma, with the use of a monoclonal antibody specific for CIAV. Expand
Molecular cloning and sequence analysis of the genome of chicken anaemia agent.
TLDR
The replicative form (RF) DNA of chicken anaemia agent (CAA) was isolated and cloned into bacterial plasmids, and an antigen was produced which gave positive immunofluorescence when detected with an anti-CAA serum. Expand
Detection of chicken anaemia agent in chickens by immunofluorescence and immunoperoxidase staining.
TLDR
An immunofluorescent method for the detection of CAA antigens in impression smears and cryostat sections of bone marrow and thymus tissue from infected birds is described and both rabbit and chicken antisera to the virus were detected. Expand
Characterization of proteins of chicken infectious anemia virus with monoclonal antibodies.
TLDR
Eight monoclonal antibodies against chicken infectious anemia virus (CIAV) were developed and three viral proteins--VP1, VP2, and VP3--with estimated sizes of 45, 30, and 16 kilodaltons (kd) were immunoprecipitated using the MAbs and Cux-1-infected cell lysates. Expand
Biological characterisation of Australian isolates of chicken anaemia agent.
TLDR
Three Australian isolates of chicken anaemia agent resisted treatment at 70 degrees C for 5 min and chloroform treatment, and were indistinguishable from the reference Cux-1 and Gifu-1 isolates in cross-immunofluorescence and cross-neutralisation tests employing polyclonal chicken antiserums. Expand
Chicken anaemia virus infection: molecular basis of pathogenicity.
TLDR
DNA analysis of CAV strains isolated in different continents revealed only minor differences among the various isolates, and apparently, all CAV isolates belong to a single serotype. Expand
Chicken anaemia agent: a review.
  • M. Mcnulty
  • Biology, Medicine
  • Avian pathology : journal of the W.V.P.A
  • 1991
TLDR
Chicken anaemia agent causes a syndrome in chickens characterised by increased mortality, anaemia associated with atrophy of the haematopoietic tissues in the bone marrow, subcutaneous and intramuscular haemorrhages, and atrophy in the lymphoid system. Expand
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