Purification and biochemical characterisation of endoplasmic reticulum alpha1,2-mannosidase from Sporothrix schenckiil.

@article{MoraMontes2010PurificationAB,
  title={Purification and biochemical characterisation of endoplasmic reticulum alpha1,2-mannosidase from Sporothrix schenckiil.},
  author={H{\'e}ctor M Mora-Montes and Claudia I Robledo-Ortiz and Laura C Gonz{\'a}lez-S{\'a}nchez and Adolfo L{\'o}pez-Esparza and Everardo L{\'o}pez-Romero and Arturo Flores-Carre{\'o}n},
  journal={Memorias do Instituto Oswaldo Cruz},
  year={2010},
  volume={105 1},
  pages={79-85}
}
Alpha 1,2-mannosidases from glycosyl hydrolase family 47 participate in N-glycan biosynthesis. In filamentous fungi and mammalian cells, alpha1,2-mannosidases are present in the endoplasmic reticulum (ER) and Golgi complex and are required to generate complex N-glycans. However, lower eukaryotes such Saccharomyces cerevisiae contain only one alpha1,2-mannosidase in the lumen of the ER and synthesise high-mannose N-glycans. Little is known about the N-glycan structure and the enzyme machinery… CONTINUE READING