Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris

@article{Zhou2007PurificationAB,
  title={Purification and bioactivity of exendin-4, a peptide analogue of GLP-1, expressed in Pichia pastoris},
  author={Jin Zhou and Ju Chu and Yong-hong Wang and Honggeng Wang and Ying-ping Zhuang and Si-liang Zhang},
  journal={Biotechnology Letters},
  year={2007},
  volume={30},
  pages={651 - 656}
}
Exendin-4, a peptide analogue of glucagon-like peptide-1 (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 fusion protein, expressed by methanol induction in Pichia pastoris system, was purified to homogeneity by chromatography followed by enterokinase cleavage of the fusion protein and subsequent purification of the recombinant exendin-4. Purity of the recombinant exendin-4 was 95.6%. Bioactivity assay revealed that it had glucose-lowering and… CONTINUE READING

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