Purification and analysis of RTI40, a type I alveolar epithelial cell apical membrane protein.

@article{Gonzlez1998PurificationAA,
  title={Purification and analysis of RTI40, a type I alveolar epithelial cell apical membrane protein.},
  author={R Fernando Garc{\'i}a Gonz{\'a}lez and Leland G. Dobbs},
  journal={Biochimica et biophysica acta},
  year={1998},
  volume={1429 1},
  pages={208-16}
}
RTI40 is a 40-42 kDa protein that, within the lung, is specific to the apical plasma membrane of the rat alveolar type I cell. Type I cells cover greater than 95% of the internal surface area of the lung. In this report, we describe some of the physical properties of RTI40, and its purification to homogeneity. By liquid phase isoelectric focusing, the pI of the protein is 3.0+/-0.5. In two-dimensional immunoblots, there is a 1.0 pH unit charge train, suggesting post-translational modification… CONTINUE READING