Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.

@article{Walz1987PurificationAA,
  title={Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.},
  author={Alfred Walz and Paola Peveri and Harald Aschauer and Marco Baggiolini},
  journal={Biochemical and biophysical research communications},
  year={1987},
  volume={149 2},
  pages={
          755-61
        }
}
Human blood mononuclear cells were cultured for 24 h in the presence of LPS (100 ng per 5 x 10(6) cells), and a monocyte-derived neutrophil-activating factor (NAF) was purified to apparent homogeneity from the conditioned media. The purification consisted of ammonium sulphate precipitation, gel filtration, chromatography on phosphocellulose followed by hydroxylapatite, and reversed-phase HPLC on C4 and CN-propyl columns. Amino acid sequence analysis (32 of 50 presumed residues) shows that NAF… CONTINUE READING

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