Purification and Sequence Analysis of a Novel NADP ( H )-Dependent Type III Alcohol Dehydrogenase from Thermococcus Strain AN 1 DONGHUI

@inproceedings{Li1997PurificationAS,
  title={Purification and Sequence Analysis of a Novel NADP ( H )-Dependent Type III Alcohol Dehydrogenase from Thermococcus Strain AN 1 DONGHUI},
  author={J Li and Kenneth Stevenson},
  year={1997}
}
An NADP(H)-dependent alcohol dehydrogenase was isolated from the hyperthermophilic archaeon Thermococcus strain AN1. This enzyme is a homotetramer with a subunit molecular weight of 46,700. The enzyme oxidizes a series of primary linear alcohols but not methanol. The pH and temperature optima with ethanol as the substrate are 6.8 to 7.0 and 85°C, respectively. The enzyme readily reduced acetaldehyde with NADPH as the cofactor. The gene encoding this enzyme has been cloned and sequenced. An open… CONTINUE READING