Purification and Properties of LR 1 , an Inducible DNA Binding Protein from Mammalian

  • B Lymphocytes, Mama Williams, +1 author Nancy Maizelsj
  • Published 2001
LR1 is a lipopolysaccharide-inducible B cell-specific DNA binding activity, with sites in the immunoglobulin heavy chain enhancer and switch regions. We describe the purification of this 106-kDa protein from nuclear extracts of PD31 murine pre-B cells, using three chromatographic steps. The DNA binding activity of LR1 is dependent upon phosphorylation, and… CONTINUE READING