Purification and Partial Characterization of an Acidic α-Glucan–Protein Complex from the Fruiting Body of Pleurotus sajor-caju and Its Effect on Macrophage Activation

  title={Purification and Partial Characterization of an Acidic $\alpha$-Glucan–Protein Complex from the Fruiting Body of Pleurotus sajor-caju and Its Effect on Macrophage Activation},
  author={Saranya Satitmanwiwat and Khanok Ratanakhanokchai and Natta Laohakunjit and Patthra Pason and Chakrit Tachaapaikoon and Khin Lay Kyu},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={1884 - 1890}
The aim of this study was to purify an acidic α-glucan-protein complex from the fruiting bodies of Pleurotus sajor-caju by using the cell wall-degrading enzymes, xylanase and cellulase. The acidic glucan-protein complex was separated from a polysaccharide extract by using DEAE Toyopearl 650M anion-exchange and Sepharose CL-6B chromatography. Its homogeneity was ensured by high-performance size-exclusion chromatography and agarose gel electrophoresis. The acidic glucan-protein complex had a… 

Paenibacillus curdlanolyticus Strain B-6 Multienzyme Complex: A Novel System for Biomass Utilization

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