Purification and Partial Characterization of an Acidic α-Glucan–Protein Complex from the Fruiting Body of Pleurotus sajor-caju and Its Effect on Macrophage Activation

@article{Satitmanwiwat2012PurificationAP,
  title={Purification and Partial Characterization of an Acidic $\alpha$-Glucan–Protein Complex from the Fruiting Body of Pleurotus sajor-caju and Its Effect on Macrophage Activation},
  author={Saranya Satitmanwiwat and Khanok Ratanakhanokchai and Natta Laohakunjit and Patthra Pason and Chakrit Tachaapaikoon and Khin Lay Kyu},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2012},
  volume={76},
  pages={1884 - 1890}
}
The aim of this study was to purify an acidic α-glucan-protein complex from the fruiting bodies of Pleurotus sajor-caju by using the cell wall-degrading enzymes, xylanase and cellulase. The acidic glucan-protein complex was separated from a polysaccharide extract by using DEAE Toyopearl 650M anion-exchange and Sepharose CL-6B chromatography. Its homogeneity was ensured by high-performance size-exclusion chromatography and agarose gel electrophoresis. The acidic glucan-protein complex had a… 
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This is an open access chapter distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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