Purification and Partial Characterization of Maize (Zea mays L.) beta-Glucosidase.

Abstract

Maize (Zea mays L.) beta-glucosidase (beta-d-glucoside glucohydrolase, EC 3.2.1.21) was extracted from the coleoptiles of 5- to 6-day-old maize seedlings with 50 millimolar sodium acetate, pH 5.0. The pH of the extract was adjusted to 4.6, and most of the contaminating proteins were cryoprecipitated at 0 degrees C for 24 hours. The pH 4.6 supernatant from… (More)

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