Purification and Kinetics of Higher Plant NADH:Nitrate Reductase.

@article{Campbell1978PurificationAK,
  title={Purification and Kinetics of Higher Plant NADH:Nitrate Reductase.},
  author={Wallace H. Campbell and J Smarrelli},
  journal={Plant physiology},
  year={1978},
  volume={61 4},
  pages={611-6}
}
Squash cotyledon (Cucurbita pepo L.) NADH:nitrate reductase (NR) was purified 150-fold with 50% recovery by a single step procedure based on the affinity of the NR for blue-Sepharose. Blue-Sepharose, which is prepared by direct coupling of Cibacron blue to Sepharose, appears to bind squash NR at the NADH site. The NR can be purified in 2 to 3 hours to a specific activity of 2 mumol of NADH oxidized/minute * milligram of protein. Corn (Zea mays L.) leaf NR was also purified to a specific… CONTINUE READING

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