Purification and Evidence for the Involvement of Tryptophan Residue at the Catalytic Site of Β-fructofuranosidase from a Thermotolerant Yeast Kluyveromyces Marxianus Ncyc 2675

β Fructofuranosidase from Kluyveromyces. marxianus NCYC 2675 was purified using hydrophobic interaction chromatography.The purified enzyme had specific activity of 2116 μmoles/min/ml. Molecular weight was determined by gel filtration and SDS-PAGE. This was further confirmed by MALDI-TOF. The protein was found to be a non glycosylated monomer of molecular… CONTINUE READING