Purification and DNA binding of the D protein, a putative resolvase of the F-factor of Escherichia coli

Abstract

The D protein encoded by plasmid mini-F promotes resolution of plasmid cointegrates or dimers of the F-factor or mini-F. In addition, two rfsF sequences are essential for this site-specific, recA-independent recombination event. The D gene was cloned into an expression vector and the gene product was overproduced in Escherichia coli and purified to… (More)
DOI: 10.1007/BF00282802

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