Purification and Characterization of an Extracellular Alkaline Serine Protease with Dehairing Function from Bacillus pumilus

@article{Huang2003PurificationAC,
  title={Purification and Characterization of an Extracellular Alkaline Serine Protease with Dehairing Function from Bacillus pumilus},
  author={Qing Huang and Yong Peng and Xin Li and Haifeng Wang and Yizheng Zhang},
  journal={Current Microbiology},
  year={2003},
  volume={46},
  pages={0169-0173}
}
An extracellular alkaline serine protease (called DHAP), produced by a Bacillus pumilus strain, demonstrates significant dehairing function. This protease is purified by hydrophobic interaction chromatography, ion exchange, and gel filtration. DHAP had a pI of 9.0 and a molecular weight of approximately 32,000 Dalton. It shows maximal activity at pH 10 and with a temperature of 55°C; the enzyme activity can be completely inhibited by phenylmethylsulfonyl fluoride (PMSF) and diisopropyl… CONTINUE READING
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